We conclude that noninvasive RFR exposure induces antitumor remodeling of the TIME, leading to inhibition of tumor progression, which provides a promising novel strategy for TIME priming and potential combination with cancer immunotherapy.
An attempt to wake up the medical community to accept research done in the last 100 years proving that electromagnetic energy can replace brutal chemotherapy. Photo taken by a professional photographer, of his own daughter being treated for Neuroblastoma. The power of the image encouraged Andy to share it with others in order to highlight the 'real' face of childhood cancer. She died. The average cost for such treatment is in the order of 500k+.
Wednesday, April 3, 2024
Tuesday, March 26, 2024
Pulsed Electromagnetic Fields (PEMFs) Trigger Cell Death and Senescence in Cancer Cells
Abstract
The currently available anti-cancer therapies, such as gamma-radiation and chemotherapeutic agents, induce cell death and cellular senescence not only in cancer cells but also in the adjacent normal tissue. New anti-tumor approaches focus on limiting the side effects on normal cells. In this frame, the potential anti-tumor properties of Pulsed Electromagnetic Fields (PEMFs) through the irradiation of breast cancer epithelial cells (MCF-7 and MDA-MB-231) and normal fibroblasts (FF95) were investigated. PEMFs had a frequency of 8 Hz, full-square wave type and magnetic flux density of 0.011 T and were applied twice daily for 5 days. The data collected showcase that PEMF application decreases the proliferation rate and viability of breast cancer cells while having the opposite effect on normal fibroblasts. Moreover, PEMF irradiation induces cell death and cellular senescence only in breast cancer cells without any effect in the non-cancerous cells. These findings suggest PEMF irradiation as a novel, non-invasive anti-cancer strategy that, when combined with senolytic drugs, may eliminate both cancer and the remaining senescent cells, while simultaneously avoiding the side effects of the current treatments.
Monday, March 11, 2024
Density Based Characterization of Mechanical Cues on Cancer Cells Using Magnetic Levitation
How interesting:
A magnetic levitation device is developed (i.e., MagDense platform) where cells are levitated in a magnetic gradient allowing them to equilibrate to a levitation height that corresponds to their unique cellular density. In application of this system, MDA-MB-231 breast and A549 lung cancer cells are cultured and overall differences in cell density are observed in response to increasing collagen fiber density.
Monday, February 26, 2024
Red light therapy improves glucose metabolism
You may wonder why I post an article about light therapy, it's because light is another form of electromagnetism:
The red light can penetrate through the skin and positively impact the mitochondria within the body’s cells, helping to create more energy and allowing the cells to function better and repair themselves.
Co-lead study author Dr. Michael Powner, senior lecturer in neurobiology in the School of Health & Psychological Sciences at City University London said they decided to look at red light therapy as a way to help control blood sugar levels after reading a study from 2019Trusted Source highlighting that sunlight exposure could correlate with improved glucose metabolism.
“We explored this in bumblebees nd found that red light reduced systemic glucose after feeding,” Dr. Powner told Medical News Today. “This latest study clearly shows a translation of this to humans.”
“Red light is absorbed by mitochondria and helps them produce more energy,” he explained.
“It lubricates the energy-making machine. But in producing more energy this way they need more raw material and this is largely glucose. They take this out of the blood.”
Tuesday, January 30, 2024
Infoceuticals and imprinting may exert positive biological effects on cellular physiology.
Abstract:
Living organisms, from the simplest organization to the more complex, all share the requirement
of energy in order to perform basic functions that sustain life and ensure its continuity. Cellular
physiology requires complex mechanisms to maintain homeostasis that are activated when
normal parameters change. Ancient medical practices like acupuncture suggest that many of the
human health impairments occur because of an energetic imbalance. In this regard, NES Health
has developed the concept of "Infoceuticals" -- remedies designed to restore the body's energetic
balance, or the means by which it is powered and communicates wirelessly. These remedies may
be administered through a special preparation of mineralized water ("imprinted" with photons
and magnetic fields), or imprinted directly into the body via electronic equipment. In this report,
we used a multicellular platform to determine the effect on cellular physiology of diverse
Infoceuticals both in liquid form and through direct imprinting. We observed unique and cellspecific effects in terms of cellular membrane structure, mitochondrial function, oxidative stress,
and viral infection protection. These findings aim to provide basic science insights to the process
of understanding the underlying phenomena that take place after Infoceutical treatments.
Friday, January 5, 2024
Stanford School of Medicine Tumor Treating Fields
ROOT, Switzerland–(BUSINESS WIRE)– Novocure (NASDAQ: NVCR) today announced it has entered into an agreement with Stanford University to establish the Stanford School of Medicine Tumor Treating Fields (TTFields) Research Program. The program is intended to support both preclinical studies and clinical trials with TTFields, electric fields that exert physical forces to kill cancer cells via a variety of mechanisms.
Saturday, December 30, 2023
Ozone Sauna Therapy combined with PEMF
Conclusions: This pilot study suggests that a combination of OST and PEMF using the HOCATT machine could potentially represent potential therapeutic adjuncts for women with inflammatory disorders such as endometriosis.
Saturday, December 23, 2023
A new high-throughput device that measures the electrical properties of cancer cells through continuous flow electrorotation
From Tokyo:
The electrical properties of cancer cells can provide information on their cancer type, state, and drug resistance. However, conventional platforms to measure these properties are complex and can only analyze a few cells. Researchers have successfully developed a high-throughput device that measures the electrical properties of cancer cells through continuous flow electrorotation. The new platform offers a high degree of automation and can simultaneously analyze several cells.
Tuesday, December 12, 2023
Tumor-destroying sound waves receive FDA approval for liver treatment in humans
The U.S. Food and Drug Administration has approved the use of sound waves to break down tumors—a technique called histotripsy—in humans for liver treatment.
Pioneered at the University of Michigan, histotripsy offers a promising alternative to cancer treatments such as surgery, radiation and chemotherapy, which often have significant side effects. Today, FDA officials awarded clearance to HistoSonics, a company co-founded in 2009 by U-M engineers and doctors for the use of histotripsy to destroy targeted liver tissue.
A human trial underway since 2021 at the U-M Rogel Cancer Center and other locations has treated patients with primary and metastatic liver tumors via histotripsy, demonstrating the technology’s ability to meet the testing’s primary effectiveness and safety targets.
Optimization of tumor-treating field therapy for triple-negative breast cancer cells in vitro via frequency modulation
Currently, tumor-treating field (TTField) therapy utilizes a single “optimal” frequency of electric fields to achieve maximal cell death in a targeted population of cells. However, because of differences in cell size, shape, and ploidy during mitosis, optimal electric field characteristics for universal maximal cell death may not exist. This study investigated the anti-mitotic effects of modulating electric field frequency as opposed to utilizing uniform electric fields.
Saturday, December 9, 2023
New patented PEMF device
This unit introduces a spectrum of frequencies all at once, a departure from traditional methods that apply one frequency at a time.
$3+k
Thursday, October 26, 2023
Clinically used osteogenic PEMF signals moderately suppressed cancer cell growth and proliferation both in vitro and in vivo.
Introduction: Although there have been significant advances in research and treatments over the past decades, cancer remains a leading cause of morbidity and mortality, mostly due to resistance to standard therapies. Pulsed electromagnetic field (PEMF), a newly emerged therapeutic strategy, has been highly regarded as less invasive and almost safe to patients, is now a clinically accepted form to treat diseases including cancer. Breast and lung cancer are the most prevalent forms of human cancers, yet reported investigations on exploring regimes including PEMF are limited. Methods: Intended to examine the anti-tumor effects of a clinically accepted osteogenic PEMF and the possibility of including PEMF in breast and lung cancer treatments, we studied the effects of 2 PEMF signals (PMF1 and PMF2) on breast and lung cancer cell growth and proliferation, as well as the possible underline mechanisms in vitro and in vivo. Results: We found that both signals caused modest but significant growth inhibition (∼5%) in MCF-7 and A549 cancer cells. Interestingly, mice xenograft tumors with A549 cells treated by PEMF were smaller in sizes than controls. However, for mice with MCF-7 tumor implants, treatment with PMF1 resulted in a slight increase (2.8%) in mean tumor size, while PMF2 treated tumors showed a 9% reduction in average size. Furthermore, PEMF increased caspase 3/7 expression levels and percentage of annexin stained cells, indicating the induction of apoptosis. It also increased G0 by 8.5%, caused changes in the expression of genes associated with cell growth suppression, DNA damage, cell cycle arrest, and apoptosis. When cancer cells or xenograft tumors treated with combined PEMF and chemotherapy drugs, PEMF showed growth inhibition effect independent of cisplatin in A549 cells, but with added effect by pemetrexed for the inhibition of MCF-7 growth. Conclusion: Together, our data suggested that clinically used osteogenic PEMF signals moderately suppressed cancer cell growth and proliferation both in vitro and in vivo.
Monday, September 18, 2023
Quantum biological electron tunnelling
Researchers have discovered a new way to target and kill cancer cells in hard-to-treat brain tumours using electrically charged molecules to trigger self-destruction, that could be developed into a spray treatment used during surgery.
Friday, September 8, 2023
NovaCure Tumor Treating Field Phase III LUNAR trial in non-small cell lung cancer, leading to significant and clinically meaningful improvements in OS when used in conjunction with standard of care.
Although their recent Phase III Ovarian cancer trail failed, the Phase III LUNAR trial in non-small cell lung cancer, leading to significant and clinically meaningful improvements in OS when used in conjunction with standard of care.
Tuesday, May 9, 2023
Previously unknown intercellular electricity may power biology
Researchers have discovered that the electrical fields and activity that exist through a cell's membrane also exist within and around another type of cellular structure called biological condensates. Like oil droplets floating in water, these structures exist because of differences in density. Their foundational discovery could change the way researchers think about biological chemistry.
Tuesday, May 2, 2023
Tuesday, April 18, 2023
Electricity can heal even the worst kind of wounds three times faster, new study finds
The researchers noticed that electricity enabled the former to heal three times faster than the latter. “We were able to show that the old hypothesis about electric stimulation can be used to make wounds heal significantly faster,” said Asplund.
According to the study authors, an electric field act as a guide to skin cells. In the absence of current, the cells move randomly, and therefore, the process of healing is slow. However, when cells are electrically stimulated, they all align in one direction and migrate fast toward the damaged site, eventually making a wound heal more quickly.
Moreover, no side effects were noticed on the cultured wounded cells due to the electric stimulation.
Tuesday, March 7, 2023
Light-induced acceleration of intracellular delivery
Researchers have used 100 seconds of laser irradiation to generate convection currents that selectively accelerate biochemical reactions -- due to the photothermal effect -- by concentrating biofunctional molecules at the cell surface. Using this method, useful molecules can be transported into cells at concentrations a hundred to a thousand times lower than with conventional methods. Furthermore, they also succeeded in selectively introducing small molecules into intracellular organelles usually impossible at low concentrations (hundreds of pmol/L) as well as inducing cell death in targeted cells by concentrating anticancer active peptides into them at concentrations so low that they would not be conventionally effective (several tens of nmol/L).
Article: https://www.sciencedaily.com/releases/2023/03/230306101438.htm
Friday, November 4, 2022
Magnetism of a single atom’s nucleus
IBM Research article I found, very interesting:
The reason I researched this subject, was due to a crop circle I viewed on http://www.cropcircleconnector.com/:
Saturday, September 17, 2022
Magnetic susceptibility in the biological effects of magnetic field
This not only demonstrates the importance of magnetic susceptibility in the biological effects of magnetic field but also illustrates the potential application of high magnetic fields in biomedicine.
Wednesday, August 24, 2022
The plasticity of the aging brain can be selectively and sustainably exploited using repetitive and highly focalized neuromodulation
A new study suggests that mild, non-invasive electrical stimulation, applied through a cap with electrodes attached, could be enough to combat the effects of getting older and keep our memory circuits in a better and more robust shape.
Sunday, August 14, 2022
Tuesday, July 26, 2022
Separating cells via electric fields
Scientists develop new method and device to isolate single cells using electric fields
Wednesday, June 1, 2022
Signal-Induced Inhibition of Telomerase Activity in HL60 Cancer Cells by Signal Transduction Using the Biophysically Activated Regulative Molecule 31 (RM31): A Pilot Study
Abstract
In this pilot study, we report the use of a novel, patented biophysical technology, which enables intranuclear access and cell nucleus stimulation, via the signal of the biophysically activated regulative molecule 31 (RM31). RM31 is the name of an isolated natural molecule found in the human body and is involved in many cellular mechanisms. We used a specific low electromagnetic field frequency to activate the RM31 molecule, which leads to specific signal transduction, to investigate the effect of telomerase activity in HL60 cancer cells. Our results revealed a dramatic inhibition in telomerase activity, a 99.5% decrease within 72 hours, with avoidance of subsequent reactivation, due to the simultaneous inhibition of human telomerase reverse transcriptase (hTERT).
Wednesday, May 25, 2022
End-Stage Recurrent Glioblastoma Treated With a New Noninvasive Non-Contact Oncomagnetic Device
The Oncomagnetic device consists of 3 oncoscillators securely attached to an acrylonitrile butadiene styrene helmet and connected to a microprocessor-based electronic controller operated by a rechargeable battery (Figure 1). Further details regarding the device are given in the Supplementary Appendix. Based on a finite element model-based calculation of the spread of the field and the size and magnetization of the rotated diametrically magnetized neodymium magnets, we estimated that the combined effective field (at least 1 mT in strength) of the 3 oncoscillators covered the entire brain, including the upper part of the brain stem.
Wednesday, May 18, 2022
“CELEX” hypothesis of cancer metastasis
Quote:
It was back in the mid-1990s that Djamgoz first developed what he would later call the cellular excitability or “CELEX” the “CELEX” hypothesis of cancer metastasis. He identified voltage-gated sodium ion channels in many types of epithelial cancer cells that did not belong, and which led to cancer cells producing action potentials as if they were neurons or cardiomyocytes.
“These are normally inert tissues in your gut or skin,” Djamgoz pointed out. “They become hyperactive, excitable, invasive, antisocial, and it is this electrical excitability that drives the cancer cells into an invasive mode.”
The electrical excitability of cancer cells escaped notice for years because traditional methods of measurement, patch clamp, or microelectrode recordings, lacked the sensitivity to capture the signals.
“Instead of poking the cells with micro-electrodes, we want to plate the cells in a petri-dish with these gold microelectrode arrays on the bottom,” Djamgoz continued. “They are now sitting on these electrodes and buzzing with action potentials!”
Not only do cancer cells become unexpected generators of bioelectricity, but they also communicate with the nervous system, seemingly feeding off of it. In a paper published June 2020 in Biochimica et Biophysica Acta — Reviews on Cancer, Djamgoz showed that sympathetic nervous system stimulation seems to drive the early stages of cancer proliferation, while parasympathetic input drives invasiveness and metastasis.
Wednesday, May 11, 2022
Magnetic therapy enhances chemo treatment of breast cancer
The non-invasive and painless treatment has the potential to lower adverse side effects of chemotherapy, which are sometimes so severe that patients need to terminate treatment early or doctors have to reduce the chemo dosage, and this could worsen their disease.
“Our magnetic technology stimulates cellular oxygen respiration to produce energy,” said team leader Associate Professor Alfredo Franco-Obregón. “In certain cancers with elevated respiratory rates — such as breast tumours — the magnetic pulses cause the cancer cells to ‘hyperventilate’ and die. Fortunately, the healthy tissues near the cancer are able to tolerate the increased respiratory rate, without ill consequences. Therefore, the OncoFTX System is more selective for cancer than conventional chemotherapy or radiotherapy. Importantly, this therapy is localised, non-invasive and painless.
Each session of magnetic therapy involves exposing a breast tumour to a pulsed magnetic field at a strength of 3 millitesla, for one hour. This field amplitude is about 50 times greater than the amplitude of the Earth’s magnetic field, but 1000 times smaller than conventional magnetic resonance imaging. Safety and efficacy trials will determine the best treatment frequency for breast cancer patients.
Wednesday, May 4, 2022
Tumors partially destroyed with sound don't come back
Noninvasive sound technology breaks down liver tumors in rats, kills cancer cells and spurs the immune system to prevent further spread -- an advance that could lead to improved cancer outcomes in humans.
Friday, December 24, 2021
Radio-wave therapy is safe for liver cancer patients and shows improvement in overall survival, study suggests
Researchers at Wake Forest School of Medicine have shown that a targeted therapy using non-thermal radio waves is safe to use in the treatment of hepatocellular carcinoma (HCC), the most common type of liver cancer. The therapy also showed a benefit in overall survival.
Wednesday, December 1, 2021
Quantum Leap Challenge Institute for Quantum Sensing for Biophysics and Bioengineering
"Squires’ research lab is developing tools to manipulate and control quantum sensors in a biological system, including a technique that uses electric potentials as “walls” to keep the quantum sensor floating in one place without touching it. Squires expects this “arsenal” of nanoscale biophysical tools to provide new kinds of information."
Friday, October 29, 2021
Friday, July 23, 2021
Researchers shrunk a deadly glioblastoma tumor by more than a third using a helmet generating a noninvasive oscillating magnetic field that the patient wore on his head while administering the therapy in his own home.
Researchers shrunk a deadly glioblastoma tumor by more than a third using a helmet generating a noninvasive oscillating magnetic field that the patient wore on his head while administering the therapy in his own home. The 53-year-old patient died from an unrelated injury about a month into the treatment, but during that short time, 31% of the tumor mass disappeared. The autopsy of his brain confirmed the rapid response to the treatment.
Saturday, July 3, 2021
The Influence of the Extremely Low Frequency Electromagnetic Field on Clear Cell Renal Carcinoma
As part of the work, it was shown that low-frequency EMF shows an inhibitory effect on the proliferation of primary cancer cells, diminishing their migratory, invasive, and metastatic abilities. It also increases the apoptosis of cancer cells and the amount of reactive oxygen species. Based on the results of our research, we want to point up that the effect of ELF-EMF depends on a specific metabolic state or at a specific stage in the cell cycle of the cells under study.
Thursday, June 10, 2021
Friday, May 28, 2021
Thursday, December 24, 2020
Novel magnetic field effect in diamagnetic molecules
Experimenting with a magnetic field almost 1M times stronger than that of the Earth, researchers in the Scholes Group were able to modify the optoelectronic properties of model nonmagnetic organic chromophores. The modifications, according to the paper, arise from the induction of ring currents in the aromatic molecules.
For the experiment, researchers chose a model aromatic chromophore called a phthalocyanine, which has a molecular structure similar to chlorophyll -- nature's light absorber -- but with stronger absorption of visible light and higher stability. The calculations on this model phthalocyanine compound and its aggregates showed clear, magnetic field-dependent changes to phthalocyanine's ability to absorb light. These results mark the first to demonstrate magnetic field dependent changes to the absorption spectrum of diamagnetic molecules. But it wasn't until researchers applied the classical analogue of the solenoid that the experiment sharpened into clarity.
A solenoid is an electromagnetic device that effectively converts electrical and magnetic energy using conductive loops of wire arranged like a spring. With their thinking grounded in the behavior of solenoids, Kudisch said, they were able to rationalize that the increased magnetic field sensitivity they were observing in the phthalocyanine aggregates could depend on the relative arrangement of the phthalocyanine rings in the aggregate.
"Not only did this add extra validation to our computational support, but it also lent credence to this idea of coupled aromatic ring currents -- the ring currents of neighboring phthalocyanine chromophores in the aggregate have a geometry dependent on amplification of magnetic field sensitivity," said Kudisch. "Just like the solenoid."
Novel magnetic field effect in diamagnetic molecules -- ScienceDailyMonday, December 21, 2020
Magnetic Bacteria for precise delivery of chemo
Magnetic bacteria as micropumps -- ScienceDaily
"ETH Professor Schürle and her team investigated how to use a magnetic field to control these bacteria in the laboratory as a way to direct the flow of liquids in a controlled manner. In their experiments, they applied only relatively weak rotating magnetic fields to spin the bacteria along the desired directions. And with many bacteria in a swarm, it proved possible to move the fluid surrounding them. The bacteria produce an effect similar to that of a micropump, meaning they are able to move active substances present in the fluid in different directions, for example from the bloodstream into the tumour tissue. By using superimposed magnetic fields that locally reinforce or cancel each other out, this pumping activity can be confined to a small region with pinpoint accuracy, as Schürle's team has been able to show in simulations"
Wednesday, November 25, 2020
Physicists could do the 'impossible': Create and destroy magnetic fields from afar
Scientists have figured out a way to create and cancel magnetic fields from afar.
The method involves running electric current through a special arrangement of wires to create a magnetic field that looks as if it came from another source. This illusion has real applications: Imagine a cancer drug that could be delivered directly to a tumor deep in the body by capsules made of magnetic nanoparticles. It's not possible to stick a magnet in the tumor to guide the nanoparticles on their journey, but if you could create a magnetic field from outside the body that centered right on that tumor, you could deliver the drug without an invasive procedure.
The strength of a magnetic field decreases with distance from the magnet, and a theorem proven in 1842, Earnshaw's Theorem, says that it's not possible to create a spot of maximum magnetic field strength in empty space.
Another use might be improving transcranial magnetic stimulation, which uses magnets to stimulate neurons in the brain to treat depression. Being able to control magnetic fields at a distance could improve the targeting of transcranial magnetic stimulation, so that doctors could better focus on particular regions in the human brain.
Sunday, November 22, 2020
Electrochemotherapy
Friday, April 17, 2020
How hospitals, plants and stores can deal with Covid-19
https://www.sciencedaily.com/releases/2020/04/200414173251.htm
The International Ultraviolet Association (IUVA) believes that UV disinfection technologies can play a role in a multiple barrier approach to reducing the transmission of the virus causing COVID-19, SARS-CoV-2, based on current disinfection data and empirical evidence. UV is a known disinfectant for air, water and surfaces that can help to mitigate the risk of acquiring an infection in contact with the COVID-19 virus when applied correctly. "
http://www.iuva.org/COVID-19
Ultraviolet-LED Maker Demonstrates 30-Second Coronavirus Kill
https://spectrum.ieee.org/tech-talk/semiconductors/optoelectronics/ultravioletled-maker-demonstrates-30second-coronavirus-kill
Autonomous Robots Are Helping Kill Coronavirus in Hospitals
https://spectrum.ieee.org/automaton/robotics/medical-robots/autonomous-robots-are-helping-kill-coronavirus-in-hospitals
Shanghai introduces ultraviolet light to disinfect public buses
https://www.youtube.com/watch?v=SqytOuYfsnA
Thursday, March 12, 2020
Inactivation of Viruses
Link to paper
Saturday, February 15, 2020
The End
Sunday, January 19, 2020
Genocide or Science?
"Perhaps I did not mention, but have thought, that, not pursuing the electromagnetic aspect to healing cancer, compared to the common treatment of drugs and radiation, to me, seems to be the same, as genocide.
End of Line."
I do not want to post, this, but must.
Tuesday, January 7, 2020
Quantum Mechanics and Biology
The complexity of mutations during replication in the human cell has made the situation more puzzling to researchers who have made contributions in this area.
This matter, seen as the preserve of biologists, has attracted the interest of physicists who have argued that mutations may be guided by quantum mechanical phenomena such as proton tunnelling and decoherence.
This work examines previous works advanced by Physicists in this field and argues for a relationship between mutations and proton tunnelling.
Quantum mechanics has been briefly discussed in away understandable to Biologists while the cell and cancer biology is covered to the extent of understanding of Physicists.
Link to article
Tuesday, December 24, 2019
Ion-Based Cellular Signal Transmission, Principles of Minimum Information Loss, and Evolution by Natural Selection
Link to Article
Saturday, December 7, 2019
High Intensity Focused Ultrasound (HIFU) Triggers Immune Sensitization of Refractory Murine Neuroblastoma to Checkpoint Inhibitor Therapy.
"Combining HIFU with αCTLA-4 and αPD-L1 significantly enhances anti-tumor response, improving survival from 0 to 62.5%.
HIFU alone causes upregulation of splenic and lymph node NK cells and circulating IL-2, IFN-Æ”, and DAMPs, whereas immune regulators like CD4+Foxp3+, IL-10, and VEGF-A are significantly reduced.
HIFU combined with checkpoint inhibitors induced significant increases in intratumoral CD4+, CD8ɑ+, and CD8α+CD11c+ cells, CD11c+ in regional lymph nodes, and decrease in circulating IL-10 compared to untreated group.
We also report significant abscopal effect following unilateral treatment of mice with large, established bilateral tumors using HIFU and checkpoint inhibitors compared to tumors treated with HIFU or checkpoint inhibitors alone (61.1% survival, p<0.0001).
This combination treatment significantly also induces CD4+CD44+hiCD62L+low and, CD8α+CD44+hiCD62L+low population and are adoptively transferable imparting immunity, slowing subsequent de novo tumor engraftment."
Link to Article
Saturday, November 30, 2019
Possible traces of resonance signaling in the genome
Link to Article
Tuesday, November 26, 2019
KCa and Ca2 + Channels: The complex thought
Article Link
Friday, November 1, 2019
Voltage Gated Sodium Channels in Cancer and Their Potential Mechanisms of Action
Link to Article
Friday, October 18, 2019
Emerging medical applications based on non-ionizing electromagnetic fields from 0 Hz to 10 THz
Link to Article
Thursday, October 17, 2019
New Perspectives in the Treatment of Tumor Cells by Electromagnetic Radiation at Resonance Frequencies in Cellular Membrane Channels
The use of electromagnetic fields has been considered as adjuvant therapy for the treatment of cancer given that some clinical trials have shown that the irradiation of cancer cells with electromagnetic fields can slow down the disease progression.
Aims
We hypothesize that this effect could be amplified by irradiating tumor cells with electromagnetic fields having frequencies close to the natural resonant frequencies of membrane channels in tumor cells, in order to obtain a significant change of the ion flux across tumor cell membrane channels, inducing the largest harmful alteration in their cellular function.
Methods
Neuronal-like cells were used as a cell model and exposed for 6 h to electromagnetic fields at different frequencies (0, 50 Hz, 900 MHz) at the same intensity of 2 mT.
The exposure system was represented by two Helmholtz coils driven by a power amplifier in current mode and an arbitrary function generator.
FTIR spectroscopy was used to evaluate the results of the exposure.
Results
The results of this study showed that the Amide I vibration band increased in intensity with the increase of the frequency, leading us to assume that the displacement of the cell channels α-helices depends on the frequency of the applied electromagnetic fields.
Conclusion
This preliminary result leads us to plan future research aimed at searching for the natural frequencies of membrane channels in tumor cells using resonant electromagnetic fields in order to damage the cellular functions of tumor cells. Clinical trials are needed to confirm such a hypothesis derived from this physical study.
Link to Article
Thursday, October 10, 2019
Voltage-Gated Ion Channels in Cancer Cell Proliferation
Link to Article
Saturday, September 28, 2019
Response of neuroblastoma cells to RF currents as a function of the signal frequency
Capacitive-resistive electric transfer (CRET) is a non-invasive therapeutic strategy that applies radiofrequency electric currents within the 400–600 kHz range to tissue repair and regeneration. Previous studies by our group have shown that 48 h of intermittent exposure to a 570 kHz CRET signal at a subthermal density of 50 μA/mm2 causes significant changes in the expression and activation of cell cycle control proteins, leading to cycle arrest in human cancer cell cultures. The present study investigates the relevance of the signal frequency in the response of the human neuroblastoma cell line NB69 to subthermal electric treatment with four different signal frequency currents within the 350–650 kHz range.
Conclusions:
The understanding of the mechanisms underlying the ability of slowing down cancer cell growth through electrically-induced changes in the expression of proteins involved in the control of cell proliferation and apoptosis might afford new insights in the field of oncology.
Link to Article
Friday, September 27, 2019
The variable electric field of the membrane /Questioning the HodgkinHuxley model
Bernstein was the initiator of membrane theory but Hodgkin, Katz and especially Goldman sought to improve it.
The integration of the constant electric field should have allowed a better knowledge and computation of the membrane potential.
The study of the variations in the electric field that would exist through the membrane brings harsh contradictions in the hypotheses of the HH model.
Link to Article
Monday, September 16, 2019
The combined treatment of 150 kHz tumor treating fields (TTFields) and cisplatin or pemetrexed inhibits mesothelioma cells in vitro and in vivo.
Article
Friday, September 13, 2019
Electromagnetic fields alter the motility of metastatic breast cancer cells
However, directed migration controlled by extrinsically applied electrical signals is poorly understood.
Using a novel microfluidic platform, we found that metastatic breast cancer cells sense and respond to the net direction of weak (∼100 µV cm−1), asymmetric, non-contact induced Electric Fields (iEFs).
iEFs inhibited EGFR (Epidermal Growth Factor Receptor) activation, prevented formation of actin-rich filopodia, and hindered the motility of EGF-treated breast cancer cells.
The directional effects of iEFs were nullified by inhibition of Akt phosphorylation.
Moreover, iEFs in combination with Akt inhibitor reduced EGF-promoted motility below the level of untreated controls.
These results represent a step towards isolating the coupling mechanism between cell motility and iEFs, provide valuable insights into how iEFs target multiple diverging cancer cell signaling mechanisms, and demonstrate that electrical signals are a fundamental regulator of cancer cell migration.
Link to article
Capacitive-resistive electric transfer (CRET) is a non-invasive therapeutic strategy
Previous studies by our group have shown that 48 h of intermittent exposure to a 570 kHz CRET signal at a subthermal density of 50 μA/mm2 causes significant changes in the expression and activation of cell cycle control proteins, leading to cycle arrest in human cancer cell cultures.
The present study investigates the relevance of the signal frequency in the response of the human neuroblastoma cell line NB69 to subthermal electric treatment with four different signal frequency currents within the 350-650 kHz range.
Methods: Trypan blue assay, flow cytometry, immunofluorescence and immunoblot were used to study the effects of subthermal CRET currents on cell viability, cell cycle progression and the expression of several marker proteins involved in NB69 cell death and proliferation.
Results: The results reveal that among the frequencies tested, only a 448 kHz signal elicited both proapoptotic and antiproliferative, statistically significant responses.
The apoptotic effect would be due, at least in part, to significant changes induced by the 448 kHz signal in the expression of p53, Bax and caspase-3.
The cytostatic response was preceded by alterations in the kinetics of the cell cycle and in the expression of proteins p-ERK1/2, cyclin D1 and p27, which is consistent with a potential involvement of the EGF receptor in electrically induced changes in the ERK1/2 pathway.
This receives additional support from results indicating that the proapototic and antiproliferative responses to CRET can be transiently blocked when the electric stimulus is applied in the presence of PD98059, a chemical inhibitor of the ERK1/2 pathway.
Conclusion: The understanding of the mechanisms underlying the ability of slowing down cancer cell growth through electrically-induced changes in the expression of proteins involved in the control of cell proliferation and apoptosis might afford new insights in the field of oncology.
Article Link
Tuesday, September 10, 2019
Crucial functions associated with phototropism and circadian clocks
https://www.researchgate.net/publication/324885021_Magnetoreception_of_Photoactivated_Cryptochrome_1_in_Electrochemistry_and_Electron_Transfer
Monday, September 2, 2019
Changes in chromatin accessibility and consequently in the expression of anti-inflammatory mediators and in cell differentiation.
https://www.researchgate.net/publication/328304138_Specific_low_frequency_electromagnetic_fields_induce_epigenetic_and_functional_changes_in_U937_cells
Tuesday, August 27, 2019
Morphological effects of electric fields on avian erythrocytes (nucleated red blood cells)
Morphological changes include rounding and cytoplasm transparency.
It has been shown that the effect is non‐thermal.
Careful imaging and image analyses have been carried out to show that the degree of this effect is frequency‐dependent, and has a higher conversion rate at higher temperatures.
Furthermore, to better understand the mechanisms behind the morphological changes, we investigated the dedifferentiation hypothesis and performed a series of tests on avian erythrocytes including fluorescence spectroscopy for hemoglobin, and tests on human umbilical cord blood, mesenchymal stem cells, and bone marrow mesenchymal stem cells including flow‐cytometry analysis for expression of certain markers and calcium staining.
https://onlinelibrary.wiley.com/doi/10.1002/bem.22195
Rotating magnetic fields within circular arrays of RNA within bacteria ...
https://www.researchgate.net/publication/328659792_Bacterial_biophotons_as_non-local_information_carriers_Species-specific_spectral_characteristics_of_a_stress_response
Saturday, August 24, 2019
Could electromagnetic fields treat metastatic triple-negative breast cancer?
Slow electrons to combat cancer
Now scientists have been able to demonstrate that a previously little-observed effect actually plays a pivotal role: Due to a process called interatomic Coulombic decay, an ion can pass on additional energy to surrounding atoms.
This frees a huge number of electrons, with precisely the right amount of energy to cause optimal damage to the DNA of the cancer cells.
https://www.sciencedaily.com/releases/2019/08/190822101429.htm